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Infographic illustrating five steps: Detect, Analyze, Quantitate (highlighted for emphasis), Purify, Translate.

Quantitatively Analyze CAR Transduction and Expression

Avoiding tonic signaling while maintaining appropriate levels of CAR expression to engage the target antigen and drive immune cell effector function requires careful tuning of CAR expression.
Optimize CAR Expression

Measure Cell Surface Expression of CAR Molecules

 

Assess how changes made to the CAR transgene, expression vector, and transgene delivery method can impact the dynamics of CAR protein expression on the cell surface. Discover the CAR antigen density kits.
A linear graph with MFI on the Y axis and MESF on the X axis. Line slopes up and to the right with data points labeled low, low/med, med/high, and high.

Determine CAR-T Cell Transduction Efficiency

 

Measuring transduction efficiency confirms that

a sufficient number of T cells have been successfully engineered to express the CAR construct. See the CAR transduction efficiency flow panels. 
A scatter plot showing the distribution of Whitlow/218 linker expressing CAR cells.

Tools to Fine-Tune CAR Expression