Cell Signaling Technology

Applications 101: Mastering Research Techniques

Build a stronger foundation for your research and approach every experiment with confidence. Applications 101 is a live, expert-led webinar series designed specifically for graduate students, postdocs, and early-career researchers eager to master core laboratory techniques.

Across four practical sessions, Cell Signaling Technology application specialists walk through the essential techniques that power modern biological discovery: immunoassays (ELISA), immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry, and western blotting.

What You'll Gain:

  • Scientific Fundamentals: Understand the core principles and mechanics behind each methodology.

  • Protocol Optimization: Get step-by-step guidance on sample preparation, antibody selection, and experimental design.

  • Practical Troubleshooting: Learn how to eliminate background noise, prevent non-specific binding, and generate publication-quality data.
Scientific illustration of matched antibody pairs binding distinct epitopes on the same target antigen for sandwich immunoassay detection.

Part 1 | Pair Up: Comprehensive ELISA Kits, Carrier-Free Antibodies, and Matched Antibody Pairs

Discover the fundamentals of ELISA, common assay formats, and essential troubleshooting strategies. Learn how CST® ELISA kits and carrier-free matched antibody pairs can empower you to build robust sandwich immunoassays, including TR-FRET applications.

Speaker: Christina Theisgen PhD | Senior Global Technical Product Trainer

When: October 1, 2026 | 8:00 AM & 4:00 PM (CEST)

Multiplex immunofluorescence image of small intestinal tissue showing villus architecture, epithelial structures, cell nuclei, and multiple biomarker signals.

Part 2 | See the Signal: Mastering Immunohistochemistry (IHC) & Immunofluorescence (IF)

Explore essential IHC and IF workflows, from method selection and tissue preparation to antibody optimization and troubleshooting. Discover how application-validated CST antibodies ensure reproducible results and empower you to transition from single-target staining to multiplex biomarker detection on your precious samples.

Speaker: Flora Guarnotta MSc | Field Application Specialist

When: October 29, 2026 | 8:00 AM & 4:00 PM (CET)

3D scientific illustration of fluorescently labelled cells showing biomarker expression for multiparameter flow cytometry analysis.

Part 3 | Go with the Flow: Mastering Intracellular & Phospho-Flow Cytometry

Master the fundamentals of flow cytometry, including fluorophore selection, intracellular staining, and phospho-flow protocols. We will show you how optimizing your fixation, permeabilization, and panel design can improve reproducibility and enable you to effectively analyze complex signaling pathways.

Speaker: Yannick Nossin PhD | Senior Field Application Scientist

When: December 3, 2026 | 8:00 AM & 4:00 PM (CET)

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with NF-κB or NF-κB plus CBP, using Acetyl-NF-κB (Lys310) Antibody. Western blots were competed with acetylated peptide (middle) or non-acetylated peptide (right).

Part 4 | Band Together: Clean and Reproducible Western Blots

Master every step of the western blot workflow, from gel electrophoresis and transfer to blocking and signal detection. Learn how step-by-step best practices, effective troubleshooting, and rigorously validated CST antibodies can help you prevent weak signals, non-specific bands, and excessive background noise.

Speaker: Jessica Kamphorst | Technical Support Specialist

When: January 21, 2027 | 8:00 AM & 4:00 PM (CET)

For Research Use Only. Not for Use in Diagnostic Procedures.
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