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In-depth Learning


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Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and performing flow cytometry experiments because they allow one to distinguish living cells from dead or dying cells.

 
VIDEO

Protein Expression Levels and PTM Detection by Western Blot

Western Blot Expert Srikanth Subramanian, PhD, joins this edition of Learning Lab LIVE! to discuss the importance of understanding your target, what to keep in mind when choosing samples, tools to determine protein expression levels, and tips for detecting proteins with post-translational modifications (PTMs).

PODCAST

Responsible Science Episode 1: Overview

The phrase ‘Responsible Science’ brings up a whole host of different questions. What is it referring to? Who is responsible for what? Isn’t science already super responsible? The Science:Disrupt crew speaks with Guardian contributor Stephen Buranyi. 

 

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Tech Tips

 
VIDEO

How to Sonicate Chromatin: Sonication ChIP Protocol

Chromatin fragmentation is a crucial step in the ChIP Sonication protocol. Optimizing sonication time for ideal chromatin fragment size, and following some general tips such as keeping the sample on ice, can improve your results.

 
VIDEO

Analyze Intracellular Signaling Mechanisms with Flow Cytometry

Learn how to design flow cytometry experiments that incorporate inhibitors, agonists, or antagonists to modulate signaling activities in your samples and test hypotheses about the signaling mechanisms that may play a role in your model system. 

 
VIDEO

Multiplex IHC Optimization: Antibody Order

Your fluorescent multiplex immunohistochemistry (mIHC) staining may be affected by the order in which you apply the antibodies in your panel. This video outlines strategies to achieve balanced signals for all your targets.

 

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Useful Tools

pathways

Succinct and current overviews of selected signaling pathways.

hallmarks-cancer-get-technical

This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.

phosphosite_plus
BIOINFORMATICS RESOURCE

Phosphosite Plus

Comprehensive tool for the study of protein post-translational modifications (PTMs).

hallmarks-validation-get-technical

Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.

lab_expectations

What you should expect from your time at the bench.

blog-grant-writing-get-technical

Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First".

 

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