Senescence is a stress-inducible and irreversible form of cell cycle arrest. This video briefly reviews the features of cellular senescence and introduces a three-stage workflow and practical tips for identifying senescent cells in your samples using markers.
What's the difference between crosslinking fixatives like formaldehyde, versus dehydrating fixatives like methanol and other alcohols? Each has advantages and disadvantages in terms of effects on epitopes and preserving structure at the molecular and subcellular level, and it's important to understand the effects they may have on your protein of interest. Watch here.
Protein-protein interactions can be studied using co-immunoprecipitation (co-IP). In this Tech Tip, Sarah describes co-IP experimental design, the roles of the “bait” and “prey proteins, and the how immunoprecipitation can also be applied for the study of protein-nucleic acid interactions and post-translational modificiations.
Succinct and current overviews of selected signaling pathways.
This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.
Comprehensive tool for the study of protein post-translational modifications (PTMs).
Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.
What you should expect from your time at the bench.
Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First".