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Home / Get Technical

In-depth Learning

One antibody is used in our lab so often that we each keep our own bulk stock! This blog will discuss how we use 5G10 as a readout for sample quality as well as an indicator of a well-executed IF experiment.
 
VIDEO

Highly Sensitive Post-Translationally Modified Peptide Enrichment for LC-MS Analysis

Cleaner Immunoprecipitation, Reduced Sample Requirement, and Simplified Sample Preparation for Ubiquitination and SUMOylation. Watch now.
 
VIDEO

Reasons to Try CUT&RUN to Analyze Protein-DNA Interactions

In this Learning Lab LIVE! webinar recording, CST application expert Fang Chen provides an overview of CUT&RUN, and answers common questions about using antibodies and other technical considerations in the CUT&RUN protocol.

 

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Tech Tips

 
VIDEO

How to Identify Senescence in Cells and Tissue

Senescence is a stress-inducible and irreversible form of cell cycle arrest. This video briefly reviews the features of cellular senescence and introduces a three-stage workflow and practical tips for identifying senescent cells in your samples using markers.

 
VIDEO

Formaldehyde vs. Alcohol Fixation for Immunofluorescence (IF)

What's the difference between crosslinking fixatives like formaldehyde, versus dehydrating fixatives like methanol and other alcohols? Each has advantages and disadvantages in terms of effects on epitopes and preserving structure at the molecular and subcellular level, and it's important to understand the effects they may have on your protein of interest. Watch here.

 
VIDEO

Co-IP Bait and Prey, and other applications of Immunoprecipitation (IP)

Protein-protein interactions can be studied using co-immunoprecipitation (co-IP). In this Tech Tip, Sarah describes co-IP experimental design, the roles of the “bait” and “prey proteins, and the how immunoprecipitation can also be applied for the study of protein-nucleic acid interactions and post-translational modificiations.

 

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Useful Tools

pathways

Succinct and current overviews of selected signaling pathways.

hallmarks-cancer-get-technical

This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.

phosphosite_plus
BIOINFORMATICS RESOURCE

Phosphosite Plus

Comprehensive tool for the study of protein post-translational modifications (PTMs).

hallmarks-validation-get-technical

Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.

lab_expectations

What you should expect from your time at the bench.

blog-grant-writing-get-technical

Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First".

 

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