In this Learning Lab LIVE! webinar recording, CST application expert Fang Chen provides an overview of CUT&RUN, and answers common questions about using antibodies and other technical considerations in the CUT&RUN protocol. Read more about how CUT&RUN works and find reagents for your experiments – click here.
This video describes considerations for optimizing capture and detection antibody pairs to enable consistent ELISA results. Find your next ELISA kit built with pre-optimized capture and detection antibodies.
The effort to find and validate disease-modifying therapies has not yet led to approvable agents, and there is an urgency, and understandable controversy, about how best to achieve this ideal milestone.
Protein-protein interactions can be studied using co-immunoprecipitation (co-IP). In this Tech Tip, Sarah describes co-IP experimental design, the roles of the “bait” and “prey proteins, and the how immunoprecipitation can also be applied for the study of protein-nucleic acid interactions and post-translational modificiations.
Learn how choice of antibody diluent can affect staining results in immunohistochemistry (IHC). The optimal antibody diluent supports antibody-epitope interactions and specificity, and ultimately, the strength of your staining.
Finding a multiplex flow protocol for analysis of both surface and intracellular proteins may require optimization. In this Tech Tip, Rob introduces approaches to setting up a flow protocol based on what is known about your antibody’s compatibility with fixation and permeabilization.
This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.
Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.
Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First"