Western Blot Expert Srikanth Subramanian, PhD, joins this edition of Learning Lab LIVE! to discuss the importance of understanding your target, what to keep in mind when choosing samples, tools to determine protein expression levels, and tips for detecting proteins with post-translational modifications (PTMs).
Learn how to design flow cytometry experiments that incorporate inhibitors, agonists, or antagonists to modulate signaling activities in your samples and test hypotheses about the signaling mechanisms that may play a role in your model system.
Your fluorescent multiplex immunohistochemistry (mIHC) staining may be affected by the order in which you apply the antibodies in your panel. This video outlines strategies to achieve balanced signals for all your targets.
When setting up immunohistochemistry (IHC) experiments, cell pellet controls can tell you whether the protocol is working, or needs to be adjusted. You need to confirm that your antibody is detecting the intended target, and not something else. Watch now.
Succinct and current overviews of selected signaling pathways.
This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.
Comprehensive tool for the study of protein post-translational modifications (PTMs).
Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.
What you should expect from your time at the bench.
Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First".