Multiplexing spatial imaging technologies can be used to maximize the amount of data collected in the tumor microenvironment (TME), thereby answering more questions from fewer samples.
By understanding the antibodies, fluorophores, sample types, and imaging instrumentation used to generate your data, you will be better equipped to design your immunofluorescence experiments and to get robust and reliable results.
In this Tech Tip, Sarah describes co-IP experimental design, the roles of the “bait” and “prey proteins, and the how immunoprecipitation can also be applied for the study of protein-nucleic acid interactions and post-translational modificiations.
The CST protocol for Fluorescent Multiplex Immunohistochemistry allows you to characterize 6 or more targets in one sample with flexible experimental design by employing a serial labeling strategy. In this video, Jen highlights five key protocol steps you can optimize to help get a balanced set of signals.
It's important to use an antibody diluent that works with your particular antibody, as the choice of diluent can impact the standing results. The optimal antibody diluent supports antibody-epitope interactions and specificity, and ultimately, the strength of your staining.
Succinct and current overviews of selected signaling pathways.
This Handbook presents an introduction to Weinberg and Hanahan’s 10 Hallmarks of Cancer, and identifies key pathways and targets related to each hallmark to aid in many aspects of cancer research.
Comprehensive tool for the study of protein post-translational modifications (PTMs).
Experimental reproducibility is under fierce scrutiny. This Handbook will help you determine if your antibodies have been thoroughly validated so you can achieve reproducible results.
What you should expect from your time at the bench.
Securing funding is a critical step in moving research forward. Learn how to write a grant using a detailed, step-by-step approach, outlined in this four part blog series. Start with "Writing a Grant Part 1: First Things First".