DIY conjugation kits look good on paper, but are you getting your money’s worth? Inefficiencies caused by suboptimal labeling, interference with specificity, and/or destabilization/degradation can negatively affect performance. Another drawback: loss of antibody during conjugation steps can mean finishing with significantly less functional antibody than you started with.
With Custom Conjugation Services from Cell Signaling Technology (CST), you can avoid these time-consuming pitfalls and get the full amount of antibody you pay for.
Advantages of Custom Conjugation Services from CST:
- Flexible order sizing and service level options
- Validated in biologically relevant systems and verified by SEC
- Optimized labeling to ensure best signal-to-noise ratio
- Purification of conjugates: free dye and/or antibody removal
- Consultation and technical support from our conjugation scientists
Comparison of HRP-conjugated antibody in ELISA
Comparison of HRP conjugation of Phospho-Akt (Ser473) (193H12) Rabbit mAb conjugated using CST custom conjugation method (blue) or conjugated using a competitor’s DIY HRP conjugation kit (green), performed by ELISA assay. The relationship between the protein concentration of lysate from MCF7 cells untreated (dashed lines) or treated with human insulin-like growth factor I (solid lines) and the absorbance at 450 nm is shown (left). The signal-to-noise ratio was calculated at the 0.625 mg/ml lysate concentration (right).
Comparison of PE-conjugated antibody in flow cytometry
Comparison of PE conjugation of Helios (D8W4X) XP® Rabbit mAb using CST custom conjugation method (blue) or conjugated using a competitor’s DIY PE conjugation kit (green). Flow cytometeric analysis of RL-7 (dashed lines), a low-expressing cell line, and Jurkat (solid lines), a high-expressing cell line. The calculated signal-to-noise ratio is shown (below).
Comparison of HRP-conjugated antibody in western blot
Comparison of HRP conjugation of GAPDH (D16H11) XP® Rabbit mAb using CST conjugation method or conjugated using a competitor’s DIY HRP conjugation kit. Western blot analysis of extracts from C6 and THP-1 cells were performed with concentration-matched antibody conjugates. These data demonstrate that signal specificity is better maintained with CST custom conjugation.