Techniques to Master Multiplex Fluorescent Imaging
Get the most out of your multiplex immunofluorescence (IF) experiments using any one of a few different methods.
Use primary antibodies derived from various host species and isotypes (rabbit, IgG, mouse IgG1, rat IgG2a, etc.), then probe with fluorophone-coupled secondaries specific to the host primary.
Use primary antibodies directly conjugated to a fluorophore or enzyme.
Use conjugation-ready antibodies on a multiplex imaging platform to support higher-plex imaging needs. Available conjugations include metals, fluorophores, and oligonucleotides.
CST offers a wide variety of antibodies formulated without BSA or azide–perfect for conjugations and all of your IF needs
The Secret to Multiplexing with a Single-Host Species
What's the secret to multiplexing with antibodies raised in the same host species? Isotypes. To get staining like you see here, you can use antibodies of different isotypes—all in the same well!
β3-Tubulin (E9F3E) Mouse mAb #45058 (green, Mouse IgG1 kappa)
Myelin Basic Protein (E9P7U) Mouse mAb #83683 (yellow, Mouse IgG2b)
Lamin A/C (4C11) Mouse mAb #4777 (red, Mouse IgG2a)
Streamline Your IF Experiments with Ready-to-Use Conjugates
Generate stunning data with antibodies already conjugated to fluorophores across multiple channels. Check out this fluorescent-labeled image of fixed frozen brain tissue from an amyloid mouse model of Alzheimer's disease.
The sample was first stained with Cathepsin D (E7Z4L) XP® Rabbit mAb #88239 (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using the following ready-to-use conjugated antibodies:
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