This application note gives you tips and guidelines on how to generate a DNA library with ChIP-DNA for next-generation sequencing. Topics covered include how to:
- Adjust your PCR amplification protocol based on the amount of starting ChIP-DNA
- Confirm DNA library quality before sending it off for sequencing
- Pool DNA libraries to get desired sequencing depth for each sample library
- Assess ChIP-seq data quality on a high level before starting a more in-depth analysis