Optimize DNA Library Preparation of ChIP-DNA for Next-Generation Sequencing

This application note gives you tips and guidelines on how to generate a DNA library with ChIP-DNA for next-generation sequencing. Topics covered include how to:

  • adjust your PCR amplification protocol based on the amount of starting ChIP-DNA
  • confirm DNA library quality before sending it off for sequencing
  • pool DNA libraries to get desired sequencing depth for each sample library
  • assess ChIP-seq data quality on a high level before starting more in-depth analysis